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During immobilization, skeletal muscle undergoes decreases in size and strength with concomitant atrophic and degenerative changes in slow-twitch muscle fibers. mTOR contributes to ER stress and associated apoptosis in renal tubular cells. We evaluated the position of the bottom of the superior facet and that of the superior border of the transverse process in relation to the center of the pedicle. Implementation of this method in sparse scattering specimens, such as engineered tissues, has the potential to improve cellular detection and categorization.

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